@endlesshc,
Absolutely!
LifterLMS Templates are templates like any other so anything from acf like get_field() for example will work just fine within them and courses/lessons/etc are simply custom post types so you can easily attach field groups to them.
You’ll want to override templates in your theme using our template override guide: https://lifterlms.com/docs/lifterlms-templates/#template-overrides
I see a lot of folks drop the ENTIRE templates directory into their theme. I caution against that (FROM EXPERIENCE!) it’s a lot easier to maintain what you’ve customized if you only override the files you’re actually customizing (as opposed to everything!)
Hope that helps,