Here is a way with jQuery to remove the very last ‘after’.
http://ww.wp.xz.cn/support/topic/413518
You can specify the ul id or a div with an id as mentioned in the post about multiple menus.
http://codex.ww.wp.xz.cn/Function_Reference/wp_nav_menu
This could be done using CSS. Something similar to:
#navigation a{
border-right:1px solid #000; //adds a border to the links in the menu
}
#navigation a:last-child{
border-right:0; //removes the border from the last one
}
Oops I meant:
#navigation a{
border-right:1px solid #000; //adds a border to the links in the menu
}
#navigation li:last-child a{
border-right:0; //removes the border from the last one
}