This might not work for anyone else but cazwilson09, but I was having the same problem until under Settings > Lightbox Gallery I filled out the following fields:
- In case that you would like to set the default number of columns:
- In case that you would like to set the default thumbnail size:
- In case that you would like to set the default lightbox size:
What I overlooked the first time was that in the latter two options, you must use one of the choices mentioned to the right of the field, i.e. thumbnail medium large full. Once I entered these variables, Lightbox Gallery worked great.